SERUM IRON DETERMINATION

Serum iron determination.

N IRON REAGENT, 4 ,7-diphenyl-1 , lO-phenanthroline, which has a more desirable sensitivity than other known iron reagents, has been introduced by Case (1), has been used by Smith et at. (2) in the estimation of iron in water, and in serum by Peterson (3) who reported the molar absorbency index of the iron complex of this new reagent as almost three times that of a-a’-dipyridyl or o-phenanthrol...

Serum iron determination.

A simple, rapid, and sensitive method for the determination of iron in serum or plasma is described. The procedure is carried out at room temperature with 2 ml. of serum or plasma, or with 1 ml. if high values are expected; it can be applied to turbid or jaundiced samples, whether previously frozen or not. An ethanolic solution of 4: 7-diphenyl-1: 10-phenanthroline is used to produce a coloured...

Determination of the Serum Iron-binding Capacity.

A colorimetric and an isotopic method for determining the iron-binding capacity are described, using only 1 ml. samples of plasma or serum. Both methods are simple, rapid and sensitive. Satisfactory results are obtained from frozen as well as from fresh samples.

The improved determination of iron in serum.

Cleaning the Apparatus After each series of examinations the reaction chamber is cleaned with a 1%0 solution of triethanolamine stained with phenol red; this is followed by lactic acid water. When the mercury becomes dirty and begins to form little balls in the reaction chamber I to 2 ml. of 200,, ferricyanide solution is introduced into the reaction chamber and shaken well. Lactic acid and wat...

Direct flow automated serum-iron determination